05/07/2026
Understanding Alanine Aminotransferase (ALT): From Liver Cell to Laboratory Report
Have you ever wondered what an ALT blood test measures and why it is one of the most commonly requested liver function tests?
This visual guide takes you through the complete journey, including:
âś… What ALT is and its role in liver cells
âś… How liver cell injury releases ALT into the bloodstream
âś… Blood sample collection and laboratory processing
âś… The biochemical principle of the ALT assay
âś… The coupled enzymatic reaction involving LDH and NADH
âś… How Clinical Chemistry analysers measure ALT activity
âś… The importance of quality control and Biomedical Scientist validation
âś… Clinical interpretation of ALT alongside other Liver Function Tests (LFTs)
Whether you are a Biomedical Science student, trainee Biomedical Scientist, healthcare professional or simply interested in laboratory medicine, this guide explains the science behind the test in a clear, visual format.
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This content is provided for educational purposes and should not replace professional medical advice or local laboratory procedures.
