Fe-Free

22/01/2026

Breakthrough electromagnetic solution for chronic wounds and antibiotic resistance. Over 70% bacterial reduction proven. Non-invasive, safe, and resistance-proof technology.

20/05/2025

Fe-Free: A unique electromagnetic and non-invasive technology for antibiotic resistant Infections and cancer treatment

*The solution:
Electromagnetic, innovative, non-invasive technology and a device for curing antibiotic-resistant infections that support/stimulate cancer tumors.
Non-invasive treatment lasting approximately 4 hours, painless, without substances.
The technology is based on the ability to disrupt iron consumption by bacteria throughout their entire life cycle, by causing their eradication or dispersal from the biofilm.
We will examine the medical impact of disrupting the biofilm in gastrointestinal tumors, as a complementary discipline that supports acceptable protocols.
For further information: [email protected],
www.fe-free.com

20/05/2025

Did you know that bacterial biofilm is involved in cancer development?
"Biofilms can promote cancer through various mechanisms as follows: Biofilms can trigger inflammation that often fails to clear biofilm-associated pathogens, and persistent inflammation can cause DNA damage and promote the growth of cancer cells ."

The intestine is a "house" for many beneficial bacteria. Although the good bacteria live in
biofilm and most of them cling to the intestine internal surface, they do not damage the
mucosal lining of the intestine. Dysbiosis (imbalance) of the biofilm can lead to a pe*******on
of pathogenic bacteria CRC, like the anaerobe Fusobacterium nucleatum (Fn) bacteria, which
can damage the mucosal layer of the intestine, initiating a chain of changes that promote
Colorectal cancer (CRC). Biofilm can aid in tumor initiation, establishment and metastasis
(Choi, Murray et al. 2023) .
The bacterium Helicobacter Pillory (Hp) which inhabits the stomach can act as a carcinogenic
substance, leading to gastric cancer. Especially the biofilm form of H. Pilory has been found
to be associated with cancer and some of the mechanisms leading to cellular transformation
by Hp has been elucidated. Moreover, an antibiotic used against Hp was found ineffective
against biofilm of Hp, raising the danger of letting this bacteria survive in the stomach as
biofilm.
Since bacteria are abundant in the gastric and intestine systems, it is easy to see the link
between bacteria and cancer. However, surprisingly, this is not the case in internal cancers,
and against the persistent medical dogma, bacterial components have been identified in the
extracellular matrix, as well as, inside cancer cells and even in immune cells. Intracellular
cell-wall-deficient bacteria has been detected in breast cancer (Nejman, Livyatan et al.
2020) . Other bacteria were identified in multiple internal cancers and all these bacteria
activate various cellular cancer-related pathways. Bacteria can also reduce the immune
response and even interfere with the effectiveness of antitumor drugs.
Targeting the biofilm inside cancers can be a good strategy to eradicate cancer cells. Indeed,
there are studies which show that interfering with the biofilm can reduce cancer cells (Choi,
Murray et al. 2023) . Our technology has the potential to reduce biofilm and hence reduce
cancer development. Moreover, since cancer cells need iron for growth, like bacterial cells,
our technology, which is based on prevention of iron entry into cells can reduce biofilm, but
can also reduce cancer cell growth.
Haya Friedman

Choi, E., et al. (2023).Biofilm and cancer: interactions and future directions for cancer
therapy. International Journal of Molecular Sciences 24(16): 12836.
Nejman, D., et al. (2020). The human tumor microbiome is composed of tumor
type–specific intracellular bacteria. Science 368(6494): 973-980.

02/04/2025

02/04/2025

Fe-Free is finishing Accelerator Road2, it was interesting, challenging, thank you very much!

02/04/2025

Minireview: The role of Iron in Bacterial Growth and Biofilm
Haya Friedman, Fe-Free
Many serious diseases result from antibiotic-resistant bacteria, like Prosthetic Join infection (PJI), Cystic Fibrosis (CF), or chronic wound infections. Recently, it was suggested that bacteria play an important role in colorectal cancer (El Tekle, Andreeva et al. 2024). One of the reason for persistent antibiotic-resistant infection is caused by a bacterial biofilm. The biofilm is a colony embedded in secretions of various molecules, creating a "shield" against the immune system and the pe*******on of antibiotics to the bacteria vicinity.
Bacteria as other organisms need iron for their metabolic processes. Iron exist in nature mainly as ores, but it also exist as Ferrous (Fe+2) or Ferric (Fe+3) ions. The concentration of free iron available for microbial use in natural environments is deficient and is around 1 × 10−18 mol/L, compared to the 1 × 10−6 mol/L required by most microbes. These ions in blood are attached to various low-molecular molecules (e.g., heme, glucan) or proteins (e.g., transferrin, ferritin). Iron can enter Gramnegative bacteria like P. aeruginosa by various mechanisms as seen in Figure 1 (Pandey 2023) and the predominant way is by production of Siderophores, small molecules with high affinity to Ferric ions. All the iron entry mechanisms requires the participation of a set of receptors and specialized transporters. For entry to occur, the siderophore-iron complexes or other iron complexes need to dock on the receptor(s). Our technology interferes with the docking and entry of iron into bacteria causing iron starvation.
Abbreviations: R = receptor; G = glucan; PBP = periplasmicbinding protein; EM =
extracellular moiety; OM = outer membrane; P = periplasm; CM = cytoplasmic membrane; C =
cytoplasm. FR=ferric reductase


Figure 1: Modes of iron entry into gram-negative bacteria from Shankar and Padeny 2023.
There are multiple studies showing that iron is necessary for bacterial biofilm survival and hence, iron starvation could be an effective approach to eradicating biofilms.
1. Iron is Essential for Biofilm Formation and Maintenance
Studies showed that a supplement of iron can induce biofilm and its depletion or replacement can prevent or reduce biofilm formation. The levels of iron in the lung of cystic fibrosis patients is very high (63M) (Berlutti, Morea et al. 2005). This high iron ions concentration most likely contribute to aggregation and biofilm formation since addition of  to planktonic P. aeruginosa increased the number of cells in biofilm.
Gallium is a molecule similar to iron and its supply to bacteria can block ironrelated activity and also iron entry (Kaneko, Thoendel et al. 2007). Application of gallium to planktonic P. aeruginosa bacteria reduces their growth and increases detachment of cells from biofilm (Kaneko, Thoendel et al. 2007). In addition, desferrioxamine-gallium (DFO-Ga, a gallium attached to a siderophore) prevented the formation of biofilm of P. aeruginosa in-vitro and also reduced, together with Gentamycin, an eye infection in rabbit, indicating that iron which was replaced by gallium is necessary for bacterial growth (Banin, Lozinski et al. 2008).
The removal of iron by chelators like EDTA caused the reduction of P. aeruginosa biofilm and addition of iron restored the biofilm growth (Banin, Brady et al. 2006). In addition, depletion of iron by the iron-specific chelator 2, 2-dipyridyl (500M) (DPD) reduced biofilm production.
Based on the effect of iron depletion on biofilm reduction, a new drug, Halogenated phenazines (HPs) HP-29 has been developed. This drug chelates ferric ions and increases iron-absorption genes in bacteria (Naclerio and Sintim 2021). This drug reduced methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE). Supporting the notion that all bacteria need iron for their survival.
2. Siderophores are Key Players in Biofilm Iron Acquisition
Siderophores are small versatile molecules, which have the capacity to bind iron ions. Siderophore are needed to bind ferric ions and enable their entry into the bacteria (Xie, Wei et al. 2024). The fact that many bacteria, as well as fungi, produce these molecules indicates that they fulfill an important role in pathogen growth and biofilm survival. For example, mutating the genes involved in siderophore production in Staphylococcus aureus reduced growth and virulence in-vivo (Dale, Doherty-Kirby et al. 2004).
3. Hypoferrmia- Innate Host Mechanism that Reduce Iron Levels upon Bacterial Infection
Multicellular organisms developed mechanisms that upon bacterial infection, immediately reduce iron levels in blood. Reducing iron levels hamper bacterial development (Ganz and Nemeth 2024). Hypoferrmia is achieved by Hepcidin-a hormone, controlling iron absorption from the gut.
Another innate iron reduction mechanism involves the iron binding protein transferrin. This protein can reduce bacterial and fungal infection in vivo (Lin, Pantapalangkoor et al. 2014).
To summarize, multitude of data indicate that iron is an essential nutrient for bacterial survival


REFERENCES
Banin, E., et al. (2006). "Chelator-induced dispersal and killing of Pseudomonas aeruginosa cells in a biofilm." Applied and environmental microbiology 72(3): 2064-2069.

Banin, E., et al. (2008). "The potential of desferrioxamine-gallium as an anti-Pseudomonas therapeutic agent." Proceedings of the National Academy of Sciences 105(43): 16761-16766.

Berlutti, F., et al. (2005). "Iron availability influences aggregation, biofilm, adhesion and invasion of Pseudomonas aeruginosa and Burkholderia cenocepacia." International journal of immunopathology and pharmacology 18(4): 661-670.

Dale, S. E., et al. (2004). "Role of siderophore biosynthesis in virulence of Staphylococcus aureus: identification and characterization of genes involved in production of a siderophore." Infection and immunity 72(1): 29-37.

El Tekle, G., et al. (2024). "The role of the microbiome in the etiopathogenesis of colon cancer." Annual Review of Physiology 86(1): 453-478.

Ganz, T. and E. Nemeth (2024). "Hypoferremia of inflammation: Innate host defense against infections." Blood Cells, Molecules, and Diseases 104: 102777.

Kaneko, Y., et al. (2007). "The transition metal gallium disrupts Pseudomonas aeruginosa iron metabolism and has antimicrobial and antibiofilm activity." The Journal of clinical investigation 117(4): 877-888.

Lin, L., et al. (2014). "Transferrin iron starvation therapy for lethal bacterial and fungal infections." The Journal of infectious diseases 210(2): 254-264.

Naclerio, G. A. and H. O. Sintim (2021). "Starving bacteria of iron: a potential strategy to disperse bacterial biofilms." Journal of medicinal chemistry 64(11): 7272-7274.

Pandey, S. S. (2023). "The role of iron in phytopathogenic microbe–plant interactions: Insights into virulence and host immune response." Plants 12(17): 3173.

Xie, B., et al. (2024). "Exploring the biological pathways of siderophores and their multidisciplinary applications: A comprehensive review." Molecules 29(10): 2318.